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Substrate solution is then added and color develops in proportion to the amount of Human CILP

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After incubation unbound Streptavidin-HRP is washed away during a washing step

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Human ITIH4 (Inter Alpha Trypsin Inhibitor Heavy Chain H4) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (ITIH4) Inter Alpha Trypsin Inhibitor Heavy Chain H4 ELISA Kit measures Inter alpha trypsin Inhibitor Heavy Chain H4 in samples. The plate has been pre coated with Human ITIH4 antibody. ITIH4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ITIH4 Antibody is added and binds to ITIH4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ITIH4 antibody. After