Mouse RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (Mini-Sample) Packaging:96 wells the TMB substrate (enzyme substrate)

the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue colored product that is directly proportional to the amount of leptin present

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL2

Then a biotinylated detection antibody specific for Mouse TJP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Many recombinant proteins have been engineered to express the HA tag

Mouse RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (Mini-Sample) Packaging:96 wells the TMB substrate (enzyme substrate)This Mouse RANL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (Mini Sample) uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to RANL . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RANL and Avidin Horseradish Peroxidase (HRP) conjugate are added