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Human HJV (Hemojuvelin) ELISA Kit PAI The plate has been pre-coatedThe Human (HJV) Hemojuvelin ELISA Kit measures Hemojuvelin in samples. The plate has been pre coated with Human HJV antibody. HJV present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HJV Antibody is added and binds to HJV in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HJV antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate