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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PDGFC
Then a biotinylated detection antibody specific for Human IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Human LCN1 antibody
The concentration of Chicken IL4 in the samples is then determined by comparing the OD of the samples to the standard curve
Human SASH3 (Sam and Sh3 Domain Containing 3) ELISA Kit BNSP Standards or samples are addedThe Human (SASH3) Sam and Sh3 Domain Containing 3 ELISA Kit measures SAM and SH3 Domain Containing 3 in samples. The plate has been pre coated with Human SASH3 antibody. SASH3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SASH3 Antibody is added and binds to SASH3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SASH3 antibody. After incubation unbound Streptavidin
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