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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL1e
When the sample containing IL- 37 is added to the plate
The concentration of Mouse NPAS4 in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Mouse PLA2G4A
Human KREMEN1 (Kremen Protein 1) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (KREMEN1) Kremen Protein 1 ELISA Kit measures Kremen protein 1 in samples. The plate has been pre coated with Human KREMEN1 antibody. KREMEN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KREMEN1 Antibody is added and binds to KREMEN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KREMEN1 antibody. After incubation unbound Streptavidin HRP is washed away
