Nano-Tag9 Mouse mAb SISd then added a HRP-conjugated antibody

then added a HRP-conjugated antibody specific to Human NGAL

or apoptosis

Then a biotinylated detection antibody specific for Rat EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Fumarase comes in two forms: mitochondrial and cytosolic

Nano-Tag9 Mouse mAb SISd then added a HRP-conjugated antibodyThe Nano tag is a new streptavidin binding peptide for both the purification and the detection of Nano tagged proteins. This peptide possesses nanomolar affinity for streptavidin and therefore is termed Nano tag. The nano tags have two types, Nano tag15 (MDVEAWLGARVPLVET) and Nano tag9 (MDVEAWLGAR), which bind to streptavidin with dissociation constants of 4 nM and 17 nM, respectively.