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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGF-AA
Then Streptavidin-HRP is added and binds to the Biotinylated S100A10 antibody
You can calculate the concentration of Human ELA1 in the samples by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL4
Human CD97 (CD97 antigen) ELISA Kit Packaging:96 wells The micro ELISA plate providedThe Human CD97 (CD97 antigen) ELISA Kit measures CD97 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CD97 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
