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Substrate solution is then added and color develops in proportion to the amount of Rat IKBKB
The plate has been pre-coated with Human FMR1NB antibody
a horseradish peroxidase conjugated mouse anti-Human SOD1 Antibody is added to the wells and binds to SOD1 in the sample
Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ADP/Acrp30 are added to the micro ELISA plate wells
Mouse/Rat OPN (Osteopontin) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Mouse Rat OPN (Osteopontin) ELISA Kit measures OPN in Mouse, Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti OPN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
