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The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve
The Human HE4 (epididymal protein 4) ELISA kit uses the Sandwich-ELISA principle
Then a biotinylated detection antibody specific for Rat BetaTG/PBP/CXCL7/NAP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EPO
Human Dickkopf-Related Protein 1 (DKK1) ELISA Kit Thyrotropi The concentration of 5-HT inThis ELISA kit is designed to detect Human Dickkopf Related Protein 1 (Human DKK1). The assay plate has been pre coated with mouse anti Human DKK1 monoclonal antibody. When the sample containing DKK1 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti Human DKK1 Antibody is added to the wells and binds to DKK1 in the sample. After washing the wells, substrate solutions are added,
