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And then biotinylated Rat C1QTNF1 Antibody is added and binds to C1QTNF1 in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1b
The concentration of Mouse TPM3 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rat NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat ET-2 (Endothelin 2) ELISA Kit Gp200 And then biotinylated Rat C1QTNF1The Rat (ET 2) Endothelin 2 ELISA Kit measures Endothelin 2 in Rat samples. The plate has been pre coated with Rat ET 2 antibody. ET 2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ET 2 Antibody is added and binds to ET 2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ET 2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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