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The concentration of AXIN1 in the sample was calculated by drawing a standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated PRKG2 antibody
CLTB present in the sample is added and binds to antibodies coated on the wells
The concentration of Human TWF1 in the samples is then determined by comparing the OD of the samples to the standard curve
Human FAM3B (Protein FAM3B) ELISA Kit ICAM1 The concentration of AXIN1 inThe Human FAM3B (Protein FAM3B) ELISA Kit measures FAM3B in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti FAM3B antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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