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PSPB in the sample or standard competes with a fixed amount of biotin-labeled PSPB for sites on a pre-coated antibody specific to PSPB
And then biotinylated Mouse ACE Antibody is added and binds to ACE in the sample
then it binds with HAMA bound to precoated antibody
The ground plant tissue is dissolved by the strong lysis solution and filtered to remove protein impurities and cell debris
Mouse HS-INS (High sensitive Insulin) Accquant® ELISA Kit TRFR PSPB in the sample orThe Mouse HS INS (High sensitive Insulin) Accquant ELISA Kit measures INS in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti INS antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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