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The concentration of Human PCSK1 in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Human HCRT
Then a biotinylated detection antibody specific for Rat IgG2a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FAS/CD95
Mouse CNTF (Ciliary Neurotrophic Factor) ELISA Kit Packaging:96 wells The concentration of Human PCSK1The Mouse (CNTF) Ciliary Neurotrophic Factor ELISA Kit measures Ciliary Neurotrophic Factor in Mouse samples. The plate has been pre coated with Mouse CNTF antibody. CNTF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CNTF Antibody is added and binds to CNTF in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CNTF antibody. After incubation unbound Streptavidin HRP is
