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The concentration of Zebrafish LIF in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GROa/CXCL1
only those wells that contain MCP5
Only those wells that contain Rat GH
Mouse CC16 (Clara Cell Protein 16) ELISA Kit (Small Sample) Packaging:96 wells The concentration of Zebrafish LIFThe Mouse CC16 (Clara Cell Protein 16) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse CC16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse CC16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution
