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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GMCSF
only those wells that contain Rat MIP1b
And then biotinylated Human CLRN1 Antibody is added and binds to CLRN1 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated LYN antibody
Human SDCBP (Syntenin-1) ELISA Kit HF2 Standards or samples are addedThe Human (SDCBP) Syntenin 1 ELISA Kit measures Syntenin 1 in samples. The plate has been pre coated with Human SDCBP antibody. SDCBP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SDCBP Antibody is added and binds to SDCBP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SDCBP antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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