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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG2b are added to the micro ELISA plate wells
only those wells that contain Mouse TPM3
only those wells that contain IL7
Substrate solution is then added and color develops in proportion to the amount of Human CTAG2
Human GDA (Guanine Deaminase) ELISA Kit DIETER Samples (or Standards) and HorseradishThe Human (GDA) Guanine Deaminase ELISA Kit measures Guanine deaminase in samples. The plate has been pre coated with Human GDA antibody. GDA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GDA Antibody is added and binds to GDA in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GDA antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
