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And then biotinylated Rabbit TNFA Antibody is added and binds to TNFA in the sample
it binds with RBL1 bound to precoated antibody
Antibodies in the samples bind to the antigen on the plate
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DEFb2
Mouse C4A (Complement Component 4a) ELISA Kit Packaging:96 wells And then biotinylated Rabbit TNFAThe Mouse C4A (Complement Component 4a) ELISA Kit measures C4a in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti C4a antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
