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then added a HRP-conjugated antibody specific to Human Cys-C
coli expression system and the target gene encoding Thr52-Ala118 is expressed
The concentration of Mouse 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve
After the bacteria are broken by lysozyme
Rat MTL (Motilin) ELISA Kit ELISA then added a HRP-conjugated antibodyThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre coated with Rat MTL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat MTL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme