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Calculate the concentration of RBX1 in the sample by plotting standard curve
Then a biotinylated detection antibody specific for Rabbit Ub and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Calculate the concentration of EIF5A in the sample by plotting standard curve
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of CCK-8 in the sample
Mouse BID (BH3 Interacting Domain Death Agonist) ELISA Kit Packaging:96 wells Calculate the concentration of RBX1The Mouse BID (BH3 Interacting Domain Death Agonist) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse BID. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BID and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to
