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The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 52 nm
PNPLA2 present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Porcine HADH Antibody is added and binds to HADH in the sample
the Human MMP-13 (Matrix Metalloproteinase 13) ELISA kit uses the Sandwich-ELISA principle
Rat F7 (Coagulation Factor 7) ELISA Kit Membrane Attack Complex The optical density (OD) isThe Rat (F7) Coagulation Factor 7 ELISA Kit measures Coagulation Factor 7 in Rat samples. The plate has been pre coated with Rat F7 antibody. F7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat F7 Antibody is added and binds to F7 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated F7 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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