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The concentration of Human GPR35 in the samples is then determined by comparing the OD of the samples to the standard curve
MSMB present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated SCN8A antibody
The OD value is proportional to the concentration of Rabbit ANXA5
Human EG-VEGF (Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit Cadherin family member 12 The concentration of Human GPR35The Human EG VEGF (Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human EG VEGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EG VEGF and Avidin Horseradish Peroxidase (HRP)
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