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Then Streptavidin-HRP is added and binds to the Biotinylated KCP antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GP39
Substrate solution is then added and color develops in proportion to the amount of Human OXT
The plate has been pre-coated with Human PI16 antibody
Human CA50 (CarbohydRate Antigen 50) ELISA Kit NPHS1 Then Streptavidin-HRP is added andThe Human CA50 (CarbohydRate Antigen 50) ELISA Kit measures CA50 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CA50 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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