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ENPP1 present in the sample is added and binds to antibodies coated on the wells
You can calculate the concentration of Rat CNTF in the samples by comparing the OD of the samples to the standard curve
only those wells that contain Human PLPL
And then biotinylated Human SCARA5 Antibody is added and binds to SCARA5 in the sample
Rat ERK1/2 (Extracellular Signal Regulated Kinase 1/2) ELISA Kit Packaging:96 wells ENPP1 present in the sampleThe Rat ERK1 2 (Extracellular Signal Regulated Kinase 1 2) ELISA Kit measures ERK1 2 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ERK1 2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
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