Calculate the concentration of PDGFRA in the sample by plotting standard curve
CREB is closely related in structure and function to CREM (cAMP response element modulator) and ATF-1 (activating transcription factor-1) proteins
Then Streptavidin-HRP is added and binds to the Biotinylated IL18R1 antibody
KLK 1 present in the sample is added and binds to antibodies coated on the wells
14,15-EET/DHET ELISA Kit Packaging:96 wells Calculate the concentration of PDGFRAThe 14,15 EET DHET ELISA Kit measures 14,15 EET DHET in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with 14,15 EET DHET. 14,15 EET DHET in the sample or standard competes with a fixed amount of biotin labeled 14,15 EET DHET for sites on a pre coated antibody specific to 14,15 EET DHET.