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Calculate the concentration of Lrp1 in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated WNT2B antibody
And then biotinylated Human EPB41L3 Antibody is added and binds to EPB41L3 in the sample
it binds with ATF3 bound to precoated antibody
Rat BCAN (Brevican core protein) ELISA Kit ALRH Calculate the concentration of Lrp1The Rat BCAN (Brevican core protein) ELISA Kit measures Bcan in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BCAN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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