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You can calculate the concentration of Rat sC5b-9 in the samples by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GRN
it binds with TSNAX bound to precoated antibody
Then Streptavidin-HRP is added and binds to the Biotinylated HDC antibody
Rat IL-35 (Interleukin 35) ELISA Kit GMEM You can calculate the concentrationThe Rat IL 35 (Interleukin 35) ELISA Kit measures IL 35 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 35 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.