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Substrate solution is then added and color develops in proportion to the amount of Mouse LTB4R2
Then Streptavidin-HRP is added and binds to the Biotinylated HSD17B10 antibody
SCD present in the sample is added and binds to antibodies coated on the wells
The plate has been pre-coated with Human ERFE antibody
Mouse AP36 (Apelin 36) ELISA Kit LAG1 Substrate solution is then addedThe Mouse AP36 (Apelin 36) ELISA Kit measures AP36 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with AP36. AP36 in the sample or standard competes with a fixed amount of biotin labeled AP36 for sites on a pre coated antibody specific to AP36. Free components are washed off. After adding HRP
