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the protein of interest is often expressed as a MBP-fusion protein
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CX3CR1
The assay plate has been pre-coated with mouse anti-Human E-Cadherin monoclonal antibody
The concentration of Mouse FLG in the samples is then determined by comparing the OD of the samples to the standard curve
Rat CHGB (Chromogranin B) ELISA Kit (Small Sample) IL-1RT-2 the protein of interest isThe Rat CHGB (Chromogranin B) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat CHGB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat CHGB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
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