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CIDEC present in the sample is added and binds to antibodies coated on the wells
PDIA3 present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated AP1G2 antibody
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of UCN2 in the sample
Rabbit TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) ELISA Kit Packaging:96 wells CIDEC present in the sampleThe Rabbit TIMP 4 (Tissue Inhibitors of Metalloproteinase 4) ELISA Kit measures TIMP 4 in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TIMP 4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.
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