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Then a biotinylated detection antibody specific for Mouse ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Mouse TLR4
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GAL9
The concentration of Rabbit HGF in the samples is then determined by comparing the OD of the samples to the standard curve
Rabbit Hepatocyte Growth Factor ELISA Kit Packaging:48 wells Then a biotinylated detection antibodyThe Rabbit Hepatocyte Growth Factor ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rabbit HGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rabbit HGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
