Rat C-Peptide (C-Peptide) ELISA Kit Packaging:96 wells Rat ANP in the sample

Rat ANP in the sample or standard competes with a fixed amount of Rat ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat ANP

BMPER present in the sample is added and binds to antibodies coated on the wells

The Human (CCL7) C-C Motif Chemokine 7 ELISA Kit measures C-C Motif Chemokine 7 in samples

And then biotinylated Mouse SEMA3B Antibody is added and binds to SEMA3B in the sample

Rat C-Peptide (C-Peptide) ELISA Kit Packaging:96 wells Rat ANP in the sampleThe Rat (C Peptide) C Peptide ELISA Kit measures Tumor necrosis factor in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added