Human HE4 (Epididymal Protein 4) QuickTest ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LCT

Then a biotinylated detection antibody specific for Rat NFE2L2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Substrate solution is then added and color develops in proportion to the amount of Human setd4

The plate has been pre-coated with Human CYTH2 antibody

Human HE4 (Epididymal Protein 4) QuickTest ELISA Kit Packaging:96 wells Standards or samples are addedThe Human HE4 (Epididymal Protein 4) QuickTest ELISA Kit measures HE4 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into