Human SESN3 (Sestrin-3) ELISA Kit STR1 Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Human MPIF-1/CCL23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The plate has been pre-coated with Mouse NCR1 antibody

Substrate solution is then added and color develops in proportion to the amount of Human CSTB

Calculate the concentration of ETV4 in the sample by plotting standard curve

Human SESN3 (Sestrin-3) ELISA Kit STR1 Then a biotinylated detection antibodyThe Human SESN3 (Sestrin 3) ELISA Kit measures SESN3 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SESN3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.