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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL34
The concentration of Human RGN in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Human RAB3D
Calculate the concentration of PRB2 in the sample by plotting standard curve
Sheep IL-4 (Interleukin 4) ELISA Kit Packaging:96 wells Standards or samples are addedThe Sheep IL 4 (Interleukin 4) ELISA Kit measures IL 4 in Sheep Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
