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CCR4 present in the sample is added and binds to antibodies coated on the wells
a horseradish peroxidase conjugated mouse anti-Human IL-7 Antibody is added to the wells and binds to IL-7 in the sample
Then a biotinylated detection antibody specific for Rat Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Human LAMP1 antibody
Human RGN (Regucalcin) ELISA Kit Packaging:96 wells CCR4 present in the sampleThe Human (RGN) Regucalcin ELISA Kit measures Regucalcin in samples. The plate has been pre coated with Human RGN antibody. RGN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RGN Antibody is added and binds to RGN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RGN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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