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Then a biotinylated detection antibody specific for Mouse αFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated SRP14 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated MAP4 antibody
it binds with WNT3A bound to precoated antibody
Rat PTH (PaRathyroid hormone) ELISA Kit GRO-A Then a biotinylated detection antibodyThe Rat PTH (PaRathyroid hormone) ELISA Kit measures PTH in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Pth antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
