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The concentration of Human GDF5 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human ENTPD1 Antibody is added and binds to ENTPD1 in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PF4
And then biotinylated Rat GRIN2A Antibody is added and binds to GRIN2A in the sample
Human PLXNA4 (Plexin A4) ELISA Kit Packaging:96 wells The concentration of Human GDF5The Human (PLXNA4) Plexin A4 ELISA Kit measures Plexin A4 in samples. The plate has been pre coated with Human PLXNA4 antibody. PLXNA4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PLXNA4 Antibody is added and binds to PLXNA4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PLXNA4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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