ALD (Aldosterone) ELISA Kit Recombinant proteins then added a HRP-conjugated antibody

then added a HRP-conjugated antibody specific to Human TIMP1

then added a HRP-conjugated antibody specific to Rat E2 to each microplate well and incubated

The concentration of Mouse IFN-Beta in the samples is then determined by comparing the OD of the samples to the standard curve

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MCP1

ALD (Aldosterone) ELISA Kit Recombinant proteins then added a HRP-conjugated antibodyThis Aldosterone ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Universal ALD. During the reaction, Universal ALD in the sample or standard competes with a fixed amount of Universal ALD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ALD. Excess conjugate and unbound sample or standard are washed away, and Avidin Horseradish Peroxidase