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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human T are added to the micro ELISA plate wells
Substrate solution is then added and color develops in proportion to the amount of Human Tnfrsf4
Calculate the concentration of NCR3 in the sample by plotting standard curve
Then a biotinylated detection antibody specific for Porcine VTN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rabbit GKN1 (Gastrokine 1) ELISA Kit Packaging:48 wells Samples (or Standards) and HorseradishThe Rabbit GKN1 (Gastrokine 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit GKN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit GKN1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate
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