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IL13 present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Mouse EPHA2
Only those wells that contain PIIINP
And then biotinylated Human ANXA4 Antibody is added and binds to ANXA4 in the sample
Human NODAL (Nodal Homolog) ELISA Kit Packaging:96 wells IL13 present in the sampleThe Human (NODAL) Nodal Homolog ELISA Kit measures Nodal homolog in samples. The plate has been pre coated with Human NODAL antibody. NODAL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NODAL Antibody is added and binds to NODAL in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NODAL antibody. After incubation unbound Streptavidin HRP is washed away during a washing
