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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S1PR2
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LDLR
Then a biotinylated detection antibody specific for Rat IFN-gamma and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
OTF present in the sample is added and binds to antibodies coated on the wells
Rat FLT1 (FMS-like Tyrosine Kinase 1) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Rat (FLT1) FMS like Tyrosine Kinase 1 ELISA Kit measures FMS like tyrosine kinase 1 in Rat samples. The plate has been pre coated with Rat Flt1 antibody. Flt1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat Flt1 Antibody is added and binds to Flt1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated Flt1 antibody. After incubation unbound Streptavidin HRP is washed away
