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The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 26 nm
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFB103B
The Human CXCL10/IP10 ELISA kit is a sandwich enzyme immunoassay
Substrate solution is then added and color develops in proportion to the amount of Human CLDN19
Human KCNIP4 (Kv Channel-interacting Protein 4) ELISA Kit Packaging:96 wells The optical density (OD) isThe Human (KCNIP4) Kv Channel interacting Protein 4 ELISA Kit measures Kv Channel interacting protein 4 in samples. The plate has been pre coated with Human KCNIP4 antibody. KCNIP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KCNIP4 Antibody is added and binds to KCNIP4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KCNIP4 antibody. After incubation unbound
