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The concentration of Rat PPARgC1a in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Human RKHD1
coli expression system and the target gene encoding Glu26-Ala189 is expressed
Then a biotinylated detection antibody specific for Human MCP-4/CCL13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat THRb (Thyroid Hormone Receptor Beta) ELISA Kit (Small Sample) ST16 The concentration of Rat PPARgC1aThe Rat THRb (Thyroid Hormone Receptor Beta) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat THRb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat THRb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
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