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LUM present in the sample is added and binds to antibodies coated on the wells
Calculate the concentration of ANG3 in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated IL17RD antibody
The plate has been pre-coated with Human SEMA3C antibody
Mouse NT5E (5'-nucleotidase) ELISA Kit GH receptor LUM present in the sampleThe Mouse NT5E (5' nucleotidase) ELISA Kit measures Nt5e in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NT5E antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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