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Substrate solution is then added and color develops in proportion to the amount of Mouse NCAM1
The plate has been pre-coated with Porcine CPB1 antibody
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EPO
Then Streptavidin-HRP is added and binds to the Biotinylated MAN1A1 antibody
Human SPON2 (Spondin-2) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (SPON2) Spondin 2 ELISA Kit measures Spondin 2 in samples. The plate has been pre coated with Human Spon2 antibody. Spon2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human Spon2 Antibody is added and binds to Spon2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated Spon2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
