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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS3/eNOS
Only those wells that contain Human VEGFR-2/KDR
The OD value is proportional to the concentration of Human E-Cad
Then a biotinylated detection antibody specific for Human TNC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Beta-actin Monoclonal Antibody (5B7)-HRP conjugated LNHR The micro ELISA plate providedBeta actin Mouse Monoclonal Antibody (5B7) HRP conjugated. Isotype: IgG1. Species Reactivity: Human, Mouse, Rat, Monkey, Canine, Chicken, Hamster, Rabbit, Pig, Sheep. Source: Mouse. Antigen: Synthetic Peptide. Composition and storage: 1 mg mL in PBS, pH 7. 4, containing 0. 02% Kathon and 50% glycerol. Store at 20C. Stable for one year from the date of shipment.
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