Fish E2 (Estradiol) ELISA Kit DPD1 The Mouse C4 (Complement Component

The Mouse C4 (Complement Component 4) ELISA Kit is a Sandwich enzyme immunoassay

The concentration of Human SHH in the samples is then determined by comparing the OD of the samples to the standard curve

The concentration of Rat CFB in the samples is then determined by comparing the OD of the samples to the standard curve

and the color intensity is directly proportional to the amount of Human CXCL9 present

Fish E2 (Estradiol) ELISA Kit DPD1 The Mouse C4 (Complement ComponentThe Fish (E2) Estradiol ELISA Kit measures Estradiol in Fish samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color develops.