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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LPAR1
The concentration of Human TNFSF9 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CNTF
Then Streptavidin-HRP is added and binds to the Biotinylated F9 antibody
Human CFL1 (Cofilin-1) ELISA Kit ABP1 The microtiter plate provided inThe Human (CFL1) Cofilin 1 ELISA Kit measures Cofilin 1 in samples. The plate has been pre coated with Human CFL1 antibody. CFL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CFL1 Antibody is added and binds to CFL1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CFL1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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