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And then biotinylated Human FCGR3B Antibody is added and binds to FCGR3B in the sample
The OD value is proportional to the concentration of Human NFE2L2
Then Streptavidin-HRP is added and binds to the Biotinylated RTN4 antibody
The concentration of Rabbit BMP4 in the samples is then determined by comparing the OD of the samples to the standard curve
Mouse ROBO4 (Roundabout Homolog 4) ELISA Kit TNFSF5 And then biotinylated Human FCGR3BThe Mouse (ROBO4) Roundabout Homolog 4 ELISA Kit measures Roundabout Homolog 4 in Mouse samples. The plate has been pre coated with Mouse ROBO4 antibody. ROBO4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ROBO4 Antibody is added and binds to ROBO4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ROBO4 antibody. After incubation unbound Streptavidin HRP is washed away
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