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Substrate solution is then added and color develops in proportion to the amount of Mouse HDAC6
Then Streptavidin-HRP is added and binds to the Biotinylated PLet1 antibody
then it binds with SCL-70 Ab bound to precoated antibody
a capture antibody-HDLC-biotin-detection antibody complex was formed
Porcine COL10 (Collagen Type X) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Porcine COL10 (Collagen Type X) ELISA Kit measures COL10 in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti COL10 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection